1- Bacteriological and plant tissue agar makes difference only in its purity. Sometimes in the plant system, these impurities present in bacteriological agar will inhibit the hormone action. our experience clearly explains this.
2- The main differences among different agar-products are due to the impurities, their level, and composition. Agar has been widely used since it has convenient gelling properties and stability during tissue culture. In all media used forin vitro culture of plants, agar is the major source of unknown variations.
You can use both bacteriological agar and plant tissue culture agar for plant tissue culture, Both types would certainly give results.
However, plant tissue culture graded agar is now available from various companies. Its always better to use it.
3- Here are some features of Bacteriological Agar
a) Gel strength of agar should be greater than 800g/cm2
b) Transparency of bacteriological agar should be lower than 10 Nephelometric turbidity units (NTU).